|
KMID : 0380620160480060618
|
|
Korean Journal of Food Science and Technology
2016 Volume.48 No. 6 p.618 ~ p.621
|
|
|
Anti-inflammatory effect of Geranium thunbergii on lipopolysaccharide-stimulated RAW 264.7 cells
|
|
Kwon Tae-Hyung
Lee Su-Jin
Kim You-Jeong
Park Jung-Ja
Kim Tae-Wan
Park Nyun-Ho
|
|
|
Abstract
|
|
|
|
Geranium thunbergii is a perennial plant commonly used as an oriental medicine for prevention of diarrhea, constipation, and gastrointestinal disorders. However, its anti-inflammatory effect has not been evaluated thus far. Therefore, the present study aimed to investigate the anti-inflammatory effect of G. thunbergii. In this study, G. thunbergii extracted with methanol; this methanol extract was further partitioned using various solvents, and G. thunbergii ethyl acetate fraction (GTEF) was obtained. To determine the anti-inflammatory activity of G. thunbergii, the effects of GTEF on nitric oxide (NO) production in lipopolysaccharide-stimulated RAW 264.7 cells were evaluated. GTEF suppressed NO production in a dose-dependent manner without any toxic effects. In addition, western blotting was performed to examine the effect of GTEF on expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggest that GTEF as a phytoextract may be useful for the prevention or treatment of inflammation.
|
|
|
KEYWORD
|
|
|
inducible nitric oxide synthase, anti-inflammatory, Geranium thunbergii, cyclooxygenase-2, plant
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|